Effect of nitrogen fixating, potassium and phosphorus solubilizing bacteria on Mungbean (Vigna radiata) yield and components yield

Document Type : Original Article


1 Deapartment of Agrotechnology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran

2 Department of Agroecology, Environmental Sciences Research Institute, Shahid Beheshti University, Tehran, Iran

3 Legume Department, Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran


Economic problems caused by increased consumption of fertilizers from one hand and environmental issues related to non-scientific consumption of such fertilizers (e.g., pollution of water and soil, loss of soil fertility, and crop quality) on the other hand, have shifted attentions towards application of biological fertilizers. In order to reduce environmental pollution and ecological damage caused by the use of chemical fertilizers, there is a need to use resources and inputs, which not only meet crop nutrient requirements, but also guarantee the long-term sustainability of agricultural systems. The purpose of this study was to reduce the harmful effects of chemical fertilizers on the agroecosystem and to reduce the cost associated with crop production through application of biofertilizers (free-living nitrogen-fixating bacteria, and potassium and phosphate solubilizing bacteria) on mung bean.
Material and methods:
A field experiment was carried out as a randomized complete block design with factorial arrangement of treatments. Two mung bean genotypes (Dezfouli and Indian) were planted under six fertilization systems at the Agricultural Research Station of Ferdowsi University of Mashhad, Iran in 2017. Fertilization treatments were Nitro Bacteria (NB), Phosphate Power Bacteria (PhPB), Potassium Power Bacteria (PPB), NB+PhPB+PPB, chemical nitrogen fertilizer (N), and no fertilizer as control (C). Prior to planting, the soil was sampled at a depth of 0-30 cm for measurement of common physico-chemical characteristics. At the end of the growing season, an area of 1 m2 from the middle of each plot was harvested for crop traits. Plant height, the number of branches per plant, grain yield, biological yield, and yield components including the number of pods per plant, the number of seeds per pod, and 100-seed weight were measured accordingly.
Results and discussion:
The results showed a significant difference between mung bean genotypes and also fertilization treatments regarding yield and yield components (p < 0.05). The highest biological yield (6555 kg ha-1 ) and grain yield (1558 kg ha-1 ) were obtained from Dezfouli genotype under NPB+PhPB+PPB treatment. The lowest biomass and grain yield were observed for Indian genotype under control treatment with 3518 and 1393 kg ha-1 , respectively. The results showed that the combined application of nitrogen stabilizing bacteria and potassium and phosphate release bacteria, due to increased access to nutrients that is an effective factor in stimulating the growth and photosynthesis of plants, improved conditions for growth and production of photosynthetic materials, and also increased the yield of mung bean plants.
It was revealed that the combined application of various biofertilizers not only improves the mung bean yield, but also can reduce negative aspects of chemical fertilizer application in crop production systems. Paying attention to more frequent use of biological fertilizers could be considered as an important agroecological approach, which results in healthier soil and water resources.


  1. Abbas, G., Abbas, Z., Aslam, M., Ullah Malik, T., Ishaque, M. and Hussain, F., 2011. Effects of organic and inorganic fertilizers on mungbean (Vigna radiata L.). International Journal of Plant Science. 2(4), 94-98.
  2. Abou, S. and Abdel, A., 2012. Impact of rock materials and biofertilizations on P and K availability for maize (Zea mays) under calcareous soil conditions. Saudi Journal of Biological Science. 19, 55-63.
  3. Adholeya, A. and Prakash A., 2004. Effect of different organic compost manures on yield and yield component of bean (Phaseolus vulgaris L). Journal of Bioresource Technology. 9(2), 311–319.
  4. Alaami Milani, M., Amini, R. and Sadegh, H., 2014. Effects of application of biofertilizers in combination with chemical fertilizers on yield and yield Grain Bean (Phaseolus vulgaris L.). Journal of Agricultural Science and Sustainable Production. 24(41), 15-29.
  5. Bashir, K., Kausar, K., Shahzad, S.M., Ashraf, M., Siddiqui, A.R., Muhammad, A. and Piracha, A., 2016. Bio-associative effect of rhizobacteria on nodulation and yield of mungbean (Vigna radiata L.) under saline conditions. Journal of Applied Agriculture and Biotechnology. 1(2), 23−37.
  6. Chattha, M.U., Hassan, U.H., Khan, E., Chattha, M.B., Ashraf, I., Ishque. W., Farooq, M., Usman, M. and Kharal, M., 2017. Effect of different nitrogen and phosphorus fertilizer levels in combination with nitrogen and phosphorus solubilizing inoculants on the growth and yield of mung bean. Journal of life Science. 15(1), 31-36.
  7. Choudhary, P., Singh, G., G Reddy, G.L. and Lal Jat. B., 2017. Effect of bio-fertilizer on different varieties of black gram (Vigna mungo L.). International Journal of Current Microbiology and Applied Sciences. 6(2), 302-316.
  8. De sousza, E.M., Bassani, V.L., Sperotto, R.A. and Granada, G.E., 2016. Inoculation of new rhizobial isilates improve nutrient uptake and growth of bean (Phaseolus vulgarid and arugula (Eruca sativa). Journal of life Science Food. 10, 258-273.
  9. Etesami, H. and Maheshwari, D., 2018. Use of plant growth promoting rhizobacteria (PGPRs) with multiple plant growth promoting traits in stress agriculture: Action mechanisms and future prospects. Journal of Ecotoxicology and Environmental Safety. 156, 225-246.
  10. Fikrettin, S., Chakmakji, R. and Kantar, F., 2004. Sugar beet and barley yield in relation to inoculation with N2-fixing and phosphate solubilizing bacteria. Journal of Plant and Soil. 256, 123-129.
  11. Hatami, H., Javidi, S. and Azizi, M., 2016. Effect of chemical, organic and biodegradable nitrogen fertilizers on yield and yield components of soybean cultivars. In Proceedings 2th International Development of Agricultural Science and Natural Resources Congress, 7th-9th December, Gorgan, Iran. (In Persian with English abstract).
  12. Hussein, A., Ali, A., Khaliq, T., Aslam, Z. and Asif, M., 2013. Growth, nodulation and yield components of mung bean of mung bean (Vigna radiata) as affected by phosphours in combination whit rhizobium inoculation. Journal of Agriculture Research. 9(30), 2319-2323.
  13. Iqbal, S., Khan, M.Y., Asghar, H.N. and Akhtar, M.J., 2016. Combined use of phosphate solubilizing bacteria and poultry manure to enhance the growth and yield of mung bean in calcareous soil. Journal of Soil Environment. 35(2), 146-15.
  14. Majnoun Hosseini, N. and Mazaheri, M., 2007. Effect of covering effects, soil mechanization systems and nitrogen fertilizer on soil properties and forage sorghum performance, 10th Iranian Soil Science Congress, 26th-28th August, Karaj, Iran. (In Persian with English abstract).
  15. Mehraban, A. and Ghasemi, A., 2014. Study of the effect of biomass and plant density on organic crop production of mushroom plant in sistan region. In Proceedings 4th International Agricultural and Sustainable Natural Resources, Congress, 13th-15th October, Tehran, Iran. (In Persian with English abstract).
  16. Navid, A., Yavarifar, A., Godini, A. and Farniya, A., 2014. Effect of nitrogenous and phosphorous biological fertilizers on morphological traits of mungbean plant. In Proceedings 4th International Agricultural and Sustainable Natural Resources, Congress, 13th-15th October, Tehran, Iran. (In Persian with English abstract).
  17. Prajapati, K., 2016. Impact of potassium solubilizing bacteria on growthand yield of mungebean vigna radiata. Journal of Applied Reseach. 6(2), 2949-555.
  18. Prasad, S.K., Singh, M.K. and Singh, J., 2014. Response of rhizobium inoculation and phosphorus levels on mungbean (Vigna radiata L.) under guava-based agri-horti system. The Bioscan. 9, 557-560.
  19. Rany, M. and Praksh, V., 2017. Effect of phosphorus, sulphur and PSB on growth attributes and yield of mungbean (Vigna radiata L.). Journal of Agriculture Search. 4(3), 198-201.
  20. Sharma, A.K., 2002. Bio-fertilizers for sustainable agriculture. Agrobios press, India.
  21. Shen, H., Xinhua He, X., Liu, Y., Chen, Y., Tang, J. and Guo, T., 2016. Complex inoculant of N2-fixing, P and K solubilizing bacteria from a purple soil improves the growth of kiwifruit (Actinidia chinensis) plantlets. Journal of Frontiers in Microbiology. 7, 1-12.
  22. Siahmargue, A., Rasisarai, M.R. and Naseri, M.Y., 2014. The effects of biological fertilizers on qualitative traits of pearl millet (Pennisetum glaucum (L.) R.Br.) forage. Journal of Plant Environmental Physiology. 9(2), 72-81.
  23. Saini, V.K., Bhandari, S.C. and Tarafder, J.C., 2004. Comparison of crop yield, soil microbial C. N. and P, N-fixation, nodulation and mycorrhizal infection in inculated and non-inoculated sorghum and chickpea crops. Field Crops Research. 89, 39-47.
  24. Venkatarao, C., Naga, S.R. and Yadav, B.L., 2017. Effect of phosphorus and biofertilizers on growth and yield of mungbean [Vigna radiata (L.) Wilczek]. International Journal of Current Microbiology. 6(7), 3992-3997.