تجزیه زیستی زایلن توسط باکتری های آزاد و تثبیتشده Sphingomonas paucimobilis strain TY4-HX ، بر اکسید گرافن

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه میکروبیولوژی، دانشکده علوم،کشاورزی وفناوری های نوین، دانشگاه ازاد اسلامی واحد شیراز، شیراز، ایران

2 گروه میکروبیولوژی، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد رشت، رشت، ایران

3 گروه فیزیک، دانشکده علوم پایه، دانشگاه آزاد اسلامی واحد مسجد سلیمان، مسجد سلیمان، ایران

چکیده

سابقه و هدف:
در چند دهه اخیر،زایلن به همراه تعدادی دیگر از ترکیب­ های آروماتیک موجود در نفت خام و دیگر محصول­ های پتروشیمی به‌عنوان یکی از آلاینده‌های مهم خاک محسوب شده­ اند، بنابراین هدف از این بررسی، یافتن سویه باکتری برای تجزیه زیستی این ترکیب و افزایش راندمان تجزیه این ترکیب به کمک تثبیت این باکتری بر روی ترکیب­ هایی با ساختار نانویی مانند اکسیدگرافن است.
مواد و روش‌ها:
در این پژوهش، تجزیه زیستی زایلن با استفاده از باکتری‌های آزاد و تثبیت‌شده به کمک اکسید گرافن در شرایط بهینه بررسی شد. باکتری‌های تجزیه‌کننده زایلن از خاک‌آلوده جداسازی شد و با استفاده از توالی ژن S rDNA 16 شناسایی گردید. که باکتری شناسایی شده Sphingomonas paucimobilis سویه TY4-HX:  بود. برای تجزیه زایلن با استفاده از سلول‌های آزاد و تثبیت‌شده ، میزان بهینه pH، دما، و غلظت زایلن به کمک روش RSM به ترتیب برابر با7، c°32وg/l  5/1 تعیین شد.
نتایج و بحث:
سلول‌های آزاد تحت شرایط بهینه قادر به تجزیه زیستی زایلن به مقدار  8/45 % طی 24 ساعت بود. در این مطالعه از اکسید گرافن به‌منظور تثبیت باکتری Sphingomonas paucimobilis سویه TY4-HX  استفاده شد. با استفاده از روش‌های FTIR و SEM مشخص شد که این سویه بااتصال به سطح اکسید گرافن قادر به ایجاد بیوفیلم شده است. باکتری‌های تثبیت‌شده به این روش قادر به تجزیه زیستی زایلن به مقدار بیش از 3/86 % تحت شرایط بهینه و در زمان 24 ساعت بود.
نتیجه‌گیری:
با توجه به نتایج به ­دست آمده، باکتری‌های تثبیت‌شده نسبت به همان سویه از باکتری‌های آزاد، از عملکرد بهتری در پالایش خاک‌آلوده به زایلن برخوردار بودند.

کلیدواژه‌ها

عنوان مقاله [English]

Xylene biodegradation by free and immobilized Sphingomonas paucimobilis strain TY4-HX on graphene oxide

نویسندگان [English]

  • Hossein Mohammadpour 1
  • Mahdi Shahriarinour 2
  • Ramin Yousefi 3
1 Department of Microbiology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2 Department of Microbiology, Faculty of Basic Sciences, Rasht Branch, Islamic Azad University, Rasht, Iran
3 Department of Physics, Masjed-Soleiman Branch, Islamic Azad University, Masjed-Soleiman, Iran
چکیده [English]

Introduction:
In recent decades, xylene has been considered as one of the most important pollutants in soil, along with other aromatic compounds in crude oil and other petrochemicals. Therefore, the aim of this study was to find bacteria for the biodegradation of this compound and to increase the degradation efficiency of this compound with the help of immobilizing the bacterium on compounds with a nanostructure such as graphene oxide.
Material and methods:
In the current study, biodegradation of xylene by free and immobilized bacteria on graphene oxide was studied under optimized conditions. Isolated xylene degrading bacteria from contaminated soils were identified based on 16S rDNA gene sequencing and submitted to gene bank as Sphingomonas paucimobilis strain TY4-HX. Using response surface methodology, optimum values of pH, temperature and xylene concentration for xylene degradation by free and immobilized cells were determined as 7, 32ºC and 1.5g/l, respectively.
Results and discussion:
Free bacterial cells were able to degrade 45.8% of the xylene after 24h under optimized conditions. Analyzes by Fourier-transform infrared spectroscopy (FTIR) and Scanning Electron Microscope (SEM) showed that the strain adhered onto the Graphene oxide surface and developed a biofilm. Immobilized cells were able to degrade up to 86.3% of the xylene after 24h under optimized conditions.
Conclusion:
Our results indicated that free and immobilized Bacteria had a suitable application potential in the treatment of xylene-containing soils.

کلیدواژه‌ها [English]

  • Biodegradation
  • Graphene oxide
  • Response surface methodology
  • SEM
  • Xylene

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